![]() ![]() ![]() Comparison of cytotoxicity effects induced by four different types of nanoparticles in human corneal and conjunctival epithelial cells. Publishing research using ab113852? Please let us know so that we can cite the reference in this datasheet.Īb113852 has been referenced in 157 publications. analyze with micro-plate reader at Ex/Em 549/575 nm, flow cytometer using 488nm laser for excitation and at emission 575 nm, or fluorescent microscope. incubate with TMRE for 15-30 min, pellet (suspension cells) / remove media (adherent cells) and wash with PBS / 0.2% BSA add FCCP to appropriate control cell samples and incubate for 10 min TMRE is suitable for the labeling of mitochondria in live cells and is not compatible with fixation. ![]() Treating cells with FCCP eliminates mitochondrial membrane potential and TMRE staining. The TMRE protocol also uses FCCP (carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone), which is a ionophore uncoupler of oxidative phosphorylation. Depolarized or inactive mitochondria have decreased membrane potential and fail to sequester TMRE. NB: TMRE is also available as free molecule as ab274305 (Tetramethylrhodamine ethyl ester). TMRE is a cell permeant, positively-charged, red-orange dye that readily accumulates in active mitochondria due to their relative negative charge. TMRE (tetramethylrhodamine, ethyl ester) is used to label active mitochondria. TMRE-Mitochondrial Membrane Potential Assay Kit ab113852 is used for quantifying changes in mitochondrial membrane potential in live cells by flow cytometry, microplate spectrophotometry and fluorescent microscopy. Store kit at 4✬ in the dark immediately upon receipt. ![]()
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